Note that items in a Column can use the Positioner attached property to access more information about its position within the Column.įor more information on using Column and other related positioner-types, see Item Positioners. If you need to perform these actions, consider positioning the items without the use of a Column. Chromatography System and controlled by Unicorn 6.3 software. High flow and low backpressure in large-scale columns High flow velocities allow increased productivity of large-scale bioprocessing operations and processing of larger volumes in one working shift. uses a Hi Trap Capto Q HP 5 ml column installed on the GE KTA pure. Also, since a Column automatically positions its children vertically, a child item within a Column should not set its y position or vertically anchor itself using the top, bottom, anchors.verticalCenter, fill or centerIn anchors. The basic characteristics of Capto S, Capto Q, and Capto DEAE are summarized in Table 1. If an item within a Column is not visible, or if it has a width or height of 0, the item will not be laid out and it will not be visible within the column.
Standardized column formats allow off-the- shelf availability for short delivery lead times. 8 m Q Strong anion 2 to 12 BSA 50 mg/mL resin Tricorn glass column Capto HiRes Q 5/50 1 mL 5 × 50 0.5 to 2.0 mL/min 4 1 column 29275878 Capto HiRes Q 10/100 8 mL 10 × 100 0.5 to 2.0 mL/min 4 1 column 29275881 S Strong cation Capto HiRes S 5/50 1 mL 5 × 50 0.5 to 2.0 mL/min 4 1 column 29275877 Capto HiRes S 10/100 8 mL 10 × 100 0.5 to 2.0. The columns are available in seven diameters with a range of BioProcess resins at three bed heights. They combine high capacity with high flow velocity and low back pressure to reduce process cycle times and increase productivity. For a 10-cm bed, a linear flow rate of 700 cm/h gives a backpressure of less than 3 bar (Fig 2). ReadyToProcess chromatography columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use. Capto S, Capto Q, and Capto DEAE are, respectively, strong cation, strong anion, and weak anion exchange resins for packed bed chromatography that increase speed and throughput in capture and intermediate purification. Compared to other chromatographic media, Capto Q has high capacity in combination with high flow velocity and low backpressure, allowing reduced process cycle times and increased productivity. Optimized for capture and intermediate protein purification. Quaternary ammonium (Q) strong anion exchanger. Suitable for screening of selectivity, binding and elution conditions, and for small-scale purifications. The combination of high volume throughput and high dynamic binding capacity for Capto Q can help increase the productivity in industrial downstream processes. HiTrap Capto Q chromatography column is prepacked with a strong anion exchange modern resin for small scale protein purification. The Column automatically positions these items in a vertical formation, like this: Capto Q is a strong anionic exchange medium. About Capto Q Capto Q resin is composed of a rigid, high-flow agarose matrix modified with dextran surface extenders and a strong quaternary ammonium (Q) anion exchanger.
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